Molecular Genetics Practical Write Up Lab Report

Molecular Genetics Practical Write Up - Lab Report Exampletyphimurium, and TA 1535, TA 1537, TA 1538 mutants.2mL of molten overlay nutrient agar-agar (kept in a 45C water-bath) was added in to a aseptic tube. After waiting for the agar to cool slightly (roughly 37C), 0.1mL of the bacterial glossiness was added exploitation a Gilson pipette fitted with a sterile tip. Immediately the molten agar was poured along with the bacterial strain onto a E-medium rest home ensuring even diffusion of the overlay agar on the plate surface. The plate was then allowed to cool before incubating at 37C overnight.2mL of molten overlay agar was added in to a sterile tube and after slightly cooling, 0.2mL 0.5mM histidine + 0.5mM biotin solution was added. 0.1 mL of the bacterial culture was added using a Gilson pipette fitted with a sterile tip and immediately the molten agar was poured along with the bacterial strain onto a E-medium plate ensuring even distribution of the overlay agar on the plate surface. The plate was then left to cool and later incubated at 37C overnight.2mL of molten overlay agar was added into a sterile tube. After the agar had slightly cooled, 0.2mL 0.5mM histidine + 0.5mM biotin solution and 0.1mL of the 0.1M histidine solution was added. Immediately pour the molten agar was poured onto a E-medium plate ensuring even distribution of the overlay agar on the plate surface and the plate allowed to cool.Six serial dilutions of the bacterial culture in strength Ringers solution ( 10-1, 10-2, 10-3, 10-4, 10-5 and 10-6) were prepared by adding, for good example for the 10-1, 100l of the bacterial culture in a sterile eppendorf tube and adding 900l of strength Ringers solution. This diluted culture was use to carry out the next dilution and repeat this was repeated six times in total in order to get the 10-6 dilution. The plate was divided into twain halves using a marker pen and marking the bottom of the plate one side was marked 10-5 and the other side 10-6. 10l of the 10-5 dilution was dropped using a Gilson